Understanding The Design Principles Of Protein Nanosensor To Combat Multidrug Resistant Enterobacteriaceae
Project Description :

Environmentally regulated gene expression is critical for bacterial survival under a variety of conditions that includes extremes in temperature, ph, osmolarity and nutrient availability. sensing the environment is orchestrated at the level of macromolecules with diverse underlying molecular mechanisms, for e.g., changes in protein oligomerization status or dna superhelicity, that eventually result in the repression or expression of genes in a finely tuned manner. however, there is little mechanistic evidence at the molecular level as to how bacteria that infect human gut employ the constant body temperature as a thermodynamic variable to regulate the expression of virulence factors. in this work, we focus on our attempts to understand the molecular origins of thermo-sensing by cnu, one of the members of the conserved hha-family, employing a battery of experimental spectroscopic, calorimetric, hydrodynamic methods and detailed computational modeling. our combined experimental-computational study reveals the structural basis for sensing not just temperature changes but even osmolarity and ph through a subtle balance between packing, electrostatics, protonation-driven conformational equilibria and excess conformational entropy of the main chain. we believe that the exquisite structure features of cnu enables its function as an environmental nano-sensor while highlighting the critical role of functional constraints in determining folding-mechanistic behaviors. since, hha family control the expressions of virulence factors in enterobacteriaceae, this molecular transducer behaviour of cnu can be exploited as a suitable drug target for controlling multi-drug resistant bacterial infections.

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Project Details :
  • Date : Oct 07,2017
  • Innovator : Abhishek Narayan
  • Guide Name : Dr. Athi Narayanan N
  • University : Indian Institutes of Technology Madras
  • Submission Year : 2018
  • Category : Biological Sciences & Bioengineering
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