In the year 2014-2015, agriculture and its allied sectors has contributed 14.2% of gva. to improve the yield and protect the plant, various novel techniques were used in recent times. in yearly 1960’s green revolution paved the way to the usage of synthetic pesticides and fertilizer to protect and increase the yield of the plants. currently these synthetic pesticides are extremely pervasive in our environment since, their half life of these chemicals is much longer time. of the same period the understanding of action and mechanism of pesticides on the human health by scientist helped to reveal the exposure and enhanced risks of neurological, immune disorders and cancers. the synthetic pesticides are mainly organophosphates (op), organochlorine(oc) and carbamates(cm). several of those pesticides —famoxadone, dieldrin, oxydemeton, chlorpyrifos, dde and esfenvalerate are extremely toxic. the conventional techniques used for pesticide detection was chromatographic methods like gas chromatography (gc), high performance liquid chromatography (hplc) along with mass spectrometry (ms). they had drawbacks like expensive, need of trained technician to handle it, complex process and time consuming. to overcome the limitations in conventional methods, a novel rapid and sensitive method to determine pesticides in the food and other samples are desired. hence, this present proposal is aimed to develop a bioactive paper based sensor, assisted with a smart phone to detect pesticide contamination in food and agricultural products. the method is developed based on the colour change in the paper biosensor due to the interaction of acetylcholine esterase in the sensor and pesticide in the sample. the image of the colour change will be captured and processed in a mobile application of smart phone. the information will be shared globally through the network for traceability. the information platform on pesticide contamination can be centralized to the scientific networks and also to the public. ultimately, this method is intended to use in domestic and commercial centers especially houses and supermarkets.
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