Background: during last few decades a significant amount of research work was conducted by the researchers to generate antimicrobial coating materials for medical devices that can efficiently inhibit the bacterial attack without affecting the human body. in textile industry cotton fibres are extensively used to prepare medical and health care products, non-implantable surgical materials etc. due to its high sweat absorbing ability and comfort. but due to this high water absorbing capacity by the cotton fibre, they are very susceptible to microbial attack which causes spreading of disease, generates bad odour and injury induced infections. natural rubber latex (nrl) based products are also extensively used in biomedical applications such as surgical gloves, dental application, wound dressing patch etc. due to their high mechanical strength as well as angiogenic propertie, reduce protein adsorption, platelet adhesion and hydrophobicity. but the presence of protein frequently causes problems related to allergic reaction (type 1 allergy) in contact with human body and increases the tendency of microbial attack. during the vulcanization of nrl the presence of activators and accelerators causes type iv allergy to human skin. though some alternating curing techniques are also available like electron beam curing, peroxide induced curing but the main disadvantage of these curing processes are high production cost and high operational safety. aim: so considering the requirement of non-toxic and biodegradable coating materials in this project, we used bio-derived easily available products like chitosan (a second abundant natural polymer derived from crustacean shell having a global production of approximately 21.4 thousand metric tons in 2015) and natural rubber latex (global production 12,314 thousand tonnes in 2015) to prepare a hydrophobic and antimicrobial coating material that is able to control the bacterial growth over the cotton fibre surface and provide considerable strength to the fibre. the cotton fibre was dipped in antimicrobial natural rubber latex (amnrl) and uv cured using thiol-ene chemistry. technical advantage: this technique allows us to prepare a hydrophobically modified antimicrobial nrl coated cotton fibre which is free from toxic sensitising and allergic processing additives. in the photochemical curing process selected polyfunctional thiol and photoinitiators were also used. compare to conventional sulphur curing system it is a low energy consuming process. amnrl coated cotton fibre exhibits superior antimicrobial activity against both gram-negative and gram-positive bacteria and it is non-toxic against fibroblast cell which indicates its potential relevancy in biomedical and anti-infective textile preparation.