The innovation herein describes the development of immunochromatographic diagnostic kit based on fluorescence silica nanosensors that serves as a promising nanodiagnostic approach for detection of intact brucella antibodies in non-serological samples eliminating painful blood collection procedures. presence of bacteria such as brucella spp. in dairy products is an immense risk to the public health. point of care immunoassays are rapid in that they can quickly screen various samples in a relatively short amount of time, are sensitive, speciﬁc and offer a great advantage in accurate and fast diagnosis of infectious diseases. we have fabricated a point of care rapid diagnostic assay that employs ﬂuorescent, micellar silica nanosensors capable of speciﬁcally detecting brucella igg antibodies in non-serological samples of afﬂicted animal (milk, urine and saliva) and human samples (urine and saliva). the developed kit holds its industrial application in agricultural, biotechnological and pharmaceutical arena. the fabricated kit comprises dye loaded silica nanoparticles conjugated with lipopolysaccharides (indian patent application 3183/mum/2013) and thus confirms its uniqueness. we have successfully fabricated and evaluated the aforementioned kit. the developed immunochromatographic diagnostic test (icdt) provides a rapid, reliable, point-of-care, accurate and reproducible result in accordance with pcr and elisa results and with high sensitivity and specificity for antibodies against b. abortus. additionally, well deﬁned antigenic components and surface biomarkers of various disease causing microbes can be broadly incorporated within the purview of this technology for accurate and rapid detection of suspected bovine pathological conditions, and can largely enable rapid ﬁeld testing that can be implemented in farms and food industry.