Pulp and paper mill is a large industrial enterprise that generates a significant amount of waste water containing high concentration of lignin causing brown color and cod. one significant problem is the persistent dark brown color in the released effluent from wastewater treatment facilities of which the major contributors are lignin and it's derivatives discharge from the pulp bleaching process.biological methods for color removal are particularly attractive.among the potential biological agents white rot fungi have been receiving attention due to their efficiency and presence of diverse ligninolytic enzyme.the white rot fungi have been reported as having promising capability of decolorizing wastewater.