Injectable hydrogels have a potential advantage over various other biomaterial matrices due to their properties like minimal invasiveness, targeted delivery and ease of encapsulation of bioactive molecules and cells before gelation. the present study describes the fabrication of silk fibroin hydrogel by utilizing bombyx mori and philosomia ricini silk. to optimize the hydrogel for biomedical applications, studies like transition time analysis, model molecule release profile, scanning electron microscopy, fourier-transform infrared spectroscopy, mathematical modelling, in-vitro analysis etc. were undertaken. silk hydrogel when loaded with growth factors, cells or drugs could be injected directly in a minimally invasive manner into a defective site requiring a matrix for tissue regeneration or could be utilized for sustained release of drugs and bioactive molecules providing a safe alternative avoiding the complications of an open surgery. moreover, the cost of fabrication of silk hydrogel is much less than the available options like collagen, hyaluronic acid based products and visco supplements.