Full thickness skin injuries like third degree burns are a growing public health problem and lead to significant morbidity and mortality. there is thus a persistent requirement of efficient, safe and affordable wound care approach. bearing this in mind, we hereby report fabrication of a novel hydrogel developed using silk fibroin (sf) as biomaterial. sf being a cost effective and biocompatible material holds great potential in wound healing applications. the facile methodology of fabrication of the hydrogel involves blending of sf proteins from two silkworms namely bombyx mori and antheraea assama. the blend led to the fabrication of a stable hydrogel matrix as the two proteins possess inherent self-assembling properties eluding the requirement of an additional cross-linker. the gelation time and matrix stiffness of the hydrogel could be modulated over a range of temperature and concentrations offering flexibility in its gelation mechanism. the hydrogels well supported the proliferation of primary human dermal fibroblast culture without significant contracture suggesting potential to be developed as cell laden grafts for treatment. the sf hydrogels aided active migration of keratinocytes as demonstrated by the agarose drop assay. in vivo studies on third degree burn wound inflicted rats showed significant accelerated wound healing response over a 3-week long comparative study with collagen gel matrix. the injectable hydrogel’s in situ gelling nature could take up the wound shape and connect the skin contours circumventing the need for sutures or cross-linkers. the hydrogel matrix in addition to providing a provisional matrix in the initial stages later was seen to promote granulation tissue formation and vascularization in the full–thickness wounds. early re-epithelization was observed through analysis of keratinocyte cytokeratin markers like ck10 and ck14. further, deposition and remodeling of collagen type i and collagen type iii fibers suggested an enhanced overall tissue regeneration. all observations were compared with that of collagen gels and it indicated the establishment of this sf hydrogel to be a possible inexpensive substitute to the former for burn wound treatment.