The present invention provides a hybrid multi-layered nanofibrous scaffold and a four dimensional dynamic cell culture apparatus. the pre-existing 3d scaffolds in the market does not address the dynamic culture conditions covered in this technology and thus do not represent the in-vivo conditions as closely as the 4d scaffold proposed in this work. furthermore, the scaffold is biocompatible and operates in non-contact mode and it is precisely controlled across all 3- dimensions.the hybrid scaffold apart from providing the extracellular matrix (ecm)-like scaffold, also meticulously simulates the mechanical stress cycles that cells experience under the in-vivo conditions. for monitoring cells on a real-time basis under microscopes, the apparatus is provided with sufficient head space over the tissue culture plate for positioning a microscope lenses. the circular slot provided at the base of the apparatus perfectly accommodates cell culture plates and also enables easy transmission of light during microscopic observation without any interference of the apparatus body. cell plasticity, differentiation and cancer metastasis are determined to large extent by cell adhesion and surface proteins which are effectively recapitulated in this scaffold.the scaffold serves as a realistic and relevant platform for studying the role of cytoskeletal proteins and focal adhesion proteins in various diseases in the fields of cardiology, dermatology, metabolism, gastroenterology, oncology and orthopedics.