A portable paper based microfluidic platform for rapid diagnosis of antimicrobial susceptibility assays comprising: foldable layered paper based microfluidic platform including a base layer, a bacterial suspension layer, a drug layer and a top layer of a chromogenic substrate which changes color in accordance to the bacterial metabolic activities. all four layers of the device are clamped in order to bring the bacterial suspension in contact with the drug and bacterial activity is subsequently monitored through colorimetric change. the said platform can be used for simultaneous testing of multiple drugs on a single platform (along with 'control' study) and the detection time is faster (~5-6 hours) than the conventional testing methods. we fabricated the paper based device following an origami and printing technique. in brief, both-side printing on standard laboratory grade filter paper (whatmann grade 1) followed by heating at 200 degree celcius for ~5 minutes was done to allow the formation of hydrophobic barriers on paper surface. while heating, the embedded toner particles were melted, thereby blocking the pores of the paper matrix. we use origami technique for layer-based fabrication of three dimensional paper devices. our device consisted of two different layers. first layer contained overnight grown bacterial cell suspension (~1-2 microlitre), whereas the second layer contain 1 microlitre of each of antibiotic and resazurin dye solutions. thereafter, the device was clamped in order to bring both the layers in close proximity, thus the bacterial growth could be monitored in presence of drugs. finally, the bacterial growth was detected through a colorimetric analysis method, i.e., by measuring the average intensity of the colorimetric signals. it is important to mention that the aforesaid device contains eight detection chambers including one chamber for ‘control’ experiment along with seven other detection pads. most of the prevalent and advanced techniques follow kirby-bauer method, which take ~18-24 hours to produce the results. herein, we followed a different modality i.e., we allowed the device to be incubated at humidified environment; which eventually lead to a faster detection, around ~5-6 hours. in a brief note, we delineate a frugal alternative for diagnosing microbial activity of a particular bacterial strain towards multiple drugs on a single device. the portable nature and ease of fabrication method collectively make it more convenient for functioning from resource limited places.